Dr. Hariharan P

Dr.-Hariharan-P

Dr. Hariharan P

Assistant Professor

M. Phil, Ph. D.

Department of Biotechnology Engineering

DOJ SIRMVIT

29/04/2022

Contact Faculty

Teaching Experience

03 Yrs

Industry/Research Experience

2 Year

Research Experience

10 years

Specialization

  • Microbial Biotechnology,
  • Therapeutic enzyme
  • Fermentation Technology

Research Interest

  • Microbial Biotechnology
  • Therapeutic enzyme

Research Topics

  • Production Purification and Characterization of Thrombolytic enzyme from fungi through solid and submerged fermentation processes.
  • Molecular cloning, Expression and Characterization of Thermostable Thrombolytic (blood clot dissolving) Metalloprotease from an Alkalophillic Bacillus subtilis into Pichia pastoris.
  • Application and Mechanism of Probiotics, Lantibiotics as a Functional Component in Food and Gut Health Benefits.
  • Purification and Characterization of lipase enzyme from phaseolus vulgaris (french bean) seeds and sesamum indicum (sesame seeds) and their synergistic action in medicaments for atherosclerosis.
  • Production Purification and Characterization of fibrinolytic enzyme from bacteria using agro waste as substrate by fermentation.
  • Synthesis, characterization and antibacterial studies of nanoparticles.

Courses

  • Scientific Foundations of Health
  • Microbiology Lab
  • Clinical Biochemistry Lab
  • Immunotechnology Lab
  • Fermentation Technology Lab
  • Enzyme Technology Lab

Funded Projects

  • Nil

Research Publications

      • Hariharan P and Chandrashekhar Naik 2021 “ Production Purification and Characterization of Thrombolytic Metalloprotease from Cladosporium Species by fermentation processes” Frontiers  “Advances in the Therapeutic Targeting of Human Matrix Metalloproteinases in Health and Disease” Abstract accepted, Manuscript Submitted.
      • Hariharan P “Optimization of media for the enhanced production of thrombolytic enzyme by newly isolated fungi Cladosporium flabelliforme MVIT CH3” Journal of Applied Microbiology and Biotechnology, Springer Nature. (Research Paper is Under Review)
      • Hariharan P “Production, Purification and Characterization of thrombolytic enzyme from Actinomycetes” Journal of Archives in Clinical Microbiology. (Research Paper is Under Review)
      • Hariharan P, Lalitha Kavya Kosuru, Nagendra H G 2022, “Purification and Characterization of lipase enzyme from phaseolus vulgaris (french bean) seeds and sesamum indicum (sesame seeds) and their synergistic action in medicaments for atherosclerosis” International Journal of Bio-Technology and Research (IJBTR) ISSN (P): 2249-6858; ISSN (E): 2249-796X Vol. 12 Issue 1, Jun 2022, 1–10
      • Sampath Kumar a, V. Shantha a, Chandrashekhar Naik b, C. Rajendra Singh b, P. Hariharan “Synthesis, characterization and antibacterial studies of calcite-zincite nanoparticles” Materials Today: Proceedings, Elsevier Ltd https://doi.org/10.1016/j.matpr.2021.01.644 2214-7853/ 2021. All rights reserved.
      • Hariharan P, Chandrashekhar Naik, Akansha Viresh Sharma, Pooja Singh, Prerana Suresh Kumar,Azra Kausar, Revansiddappa 2018 “ Isolation, Production and purification of new thrombolytic enzyme from Cladosporium Spp.” European Journal of Biotechnology and Biosciences (2019) Vol 6; Issuse 2, 01-04 ( IF-5.44)
      • Hariharan P, Chandrashekhar Naik, Pooja vajpaye and keerthana srinivasa (2017) “Production, Purification and characterization of thrombolytic enzyme from cladosporium Sp., through solid state of fermentation”. International journal of engineering research and technology. Vol.3(7) ISSN:2278-0181. (IF-3.2)
      • Chandrashekhar Naik, Revansiddappa and Hariharan P (2012)” Potential antimicrobial activity of Bacillus subtillus isolated from marine environment of Bhatkal” World journal of Science and Technology” (2012) 2 (7):128-131.
      • Chandrashekhar Naik, Revansiddappa and Hariharan P (2014) “Production, purification and isolation of cladosporium sps as a new source of thromolytic enzyme” journal of research in biology (2014) 3(7):001-008. (IF-3.2)
      • Chandrashekhar Naik, Durgappa and Hariharan P (2014) “Production, purification and characterization fibrinolytic enzyme from Fusarium Spp.” journal of research in biology. Vol.4(5) ISSN:2278-0181 (IF-3.2).

Isolated New bacterial & Fungal Species and their16S rRNA sequence has been deposited to NCBI

      • Cladosporium flabelliforme, MVIT CH3, MH681584.1,MH681584,27-July-2018,Hariharan P and Naik
      • Saccharothrix espanaensis strain SAN24, KX098456,1041750548,06-July 2016,Naik,C., Hariharan P,Shreekant,S. and Shekhar,A.
      • Bacillus subtilis strain ,SAN15,KX098457 ,1041750549,01-JUL-2016,Naik,C., Hariharan P, Shreekant,S. and Shekhar,A.

MEMBERSHIP OF PROFESSIONAL BODIES

      • Advisory committee member in Council for science and development, Dharmapuri, Tamilnadu.
      • Research advisory committee member in B P Tech Private limited, Krishnagiri, Tamilnadu.

HONOURS & ACHIEVEMENTS

      • DBT-GOI sponsored two weeks hands on training in Molecular Techniques and Application conducted by NRCBS, Madurai Kamaraj University,
      • Three Days training program on HPLC (both analytical and preparative) conducted by PES Institute of Technology, Bangalore.
      • Two Days Training program on Research Methodologies and Latex Organized by Visvesvaraya Technological University, Belgaum.
      • DBT Sponsored five days training program on: “Hands on Training in Molecular Biological Techniques”. Organized by Department of Marine Biotechnology, Bharathidasan University,

INVITED TALKS

      • Lecture Topic – “Studies and Investigation of Cancer and current approaches for vaccine development” at International conference on recent trends in vaccine development in pharmaceutical industries on 27/04/2022 at Sri Venkateswara college of Arts and Science, Dharmapuri, Tamil Nadu.
      • Lecture Topic – “current Jobs and Entrepreneurship opportunities in biological science” at National conference on Entrepreneurship opportunities in Biological sciences on 29/04/2022 at Kamadhenu college of Arts and Science Dharmapuri Tamil Nadu.
      • Lecture Topic – “Protein Purification techniques” on 19/02/2022 at Sivakamiammal college of Arts and Science, Krishnagiri, Tamil Nadu.
      • Lecture Topic – “The Basic Principles of Downstream Bioprocessing Techniques” on 23/02/2022 at Council for Science and Development, Dharmapuri, Tamil Nadu

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